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recombinant murine ip-10 (cxcl10) (peprotech #250-16)  (PeproTech)


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    PeproTech recombinant murine ip-10 (cxcl10) (peprotech #250-16)
    Recombinant Murine Ip 10 (Cxcl10) (Peprotech #250 16), supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+murine+cxcl10/cxcl10+chemokine/pm40525671-217-4-7
    Average 90 stars, based on 1 article reviews
    recombinant murine ip-10 (cxcl10) (peprotech #250-16) - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: B3GALT4 remodels the tumor microenvironment through GD2-mediated lipid raft formation and the c-met/AKT/mTOR/IRF-1 axis in neuroblastoma.
    Article Snippet: Recombinant murine CXCL9 (100 ng/ml, PeproTech, USA), CXCL10 (50 ng/ml, PeproTech, USA), and culture supernatants from transfected cells after 48 hours of culture were added to the bottom wells.

    Recombinant:

    Article Title: Islet‐Resident Memory T Cells Orchestrate the Immunopathogenesis of Type 1 Diabetes through the FABP4‐CXCL10 Axis
    Article Snippet: Activated CD8 + T cells (1 × 10 5 cells/well) were then seeded on the porous permeable membrane in the Trans‐well Supports (Corning). .. To assess the alarming function of FABP4 +/+ T RM and FABP4 −/− T RM cells and the involvement of CXCL10, the conditioned medium of respective cells were added to the lower wells. and supplemented with recombinant murine CXCL10 (2 g mL −1 , PeproTech) or IgG as control. ..

    Article Title: Islet-Resident Memory T Cells Orchestrate the Immunopathogenesis of Type 1 Diabetes through the FABP4-CXCL10 Axis.
    Article Snippet: Activated CD8+ T cells (1 × 105 cells/well) were then seeded on the porous permeable membrane in the Trans-well Supports (Corning). .. To assess the alarming function of FABP4+/+ TRM and FABP4−/− TRM cells and the involvement of CXCL10, the conditioned medium of respective cells were added to the lower wells. and supplemented with recombinant murine CXCL10 (2 g mL−1, PeproTech) or IgG as control. ..

    Article Title: Discovery of Clinical Candidate ACT-777991, a Potent CXCR3 Antagonist for Antigen-Driven and Inflammatory Pathologies.
    Article Snippet: The CXCR3 chemokine receptor is a G proteincoupled receptor mainly expressed on immune cells from the lymphoid lineage, including activated T cells.. Binding of its inducible chemokine ligands CXCL9, CXCL10, and CXCL11 leads to downstream signaling events and the migration of activated T cells to sites of inflammation.. Herein, we report the third part of our CXCR3 antagonist program in the field of autoimmunity, culminating in the discovery of the clinical compound ACT777991 (8a).

    Article Title: RORγt agonist enhances anti-PD-1 therapy by promoting monocyte-derived dendritic cells through CXCL10 in cancers
    Article Snippet: .. 2) Different concentrations of recombinant murine CXCL10 (PeproTech, Rocky Hill, USA) or 1 mg/ml neutralizing rat anti-CXCL10 mAb (R&D Systems, Minn., USA) were added to the lower wells, and CD8 + T cells were added to the upper wells. ..

    Article Title: RORγt agonist enhances anti-PD-1 therapy by promoting monocyte-derived dendritic cells through CXCL10 in cancers.
    Article Snippet: .. 2) Different concentrations of recombinant murine CXCL10 (PeproTech, Rocky Hill, USA) or 1 mg/ ml neutralizing rat anti-CXCL10 mAb (R&D Systems, Minn., USA) were added to the lower wells, and CD8+ T cells were added to the upper wells. ..

    Article Title: CXCL10-armed oncolytic adenovirus promotes tumor-infiltrating T-cell chemotaxis to enhance anti-PD-1 therapy
    Article Snippet: Lymph nodes were isolated from C57BL/6 mice and carefully ground into single cells and then were cultured in RPMI 1640 medium supplemented with 10% FBS, 100 U/mL of penicillin, 100 mg/mL of streptomycin, soluble anti-CD3 (5 μg mL −1 ; 16–0031-86; eBioscience/Thermo Fisher Scientific, Waltham, MA), anti-CD28 (5 μg mL −1 ; 16–0281-86; eBioscience) and IL-2 (200 U mL −1 ; 212–12; Peprotech, NJ, USA) for 3 days., The chemotaxis assay was then performed in 24-well plates with Transwell permeable supports using a 5 μm polycarbonate membrane (3421; Corning Life Sciences, Tewksbury, MA, USA). .. Briefly, 500 μL of 1640 alone, the supernatant of control adenovirus (Adv-Ctrl)- or Adv-CXCL10-infected MC38-CAR or 1640 with 200 pg/mL of recombinant murine CXCL10 (rCXCL10; 250–16; Peprotech), all of which were supplemented with 10% FBS, were added to the lower well. ..

    Article Title: CXCL10-armed oncolytic adenovirus promotes tumor-infiltrating T-cell chemotaxis to enhance anti-PD-1 therapy
    Article Snippet: Isolation and culture of murine lymphocytes and the in vitro chemotaxis assay Lymph nodes were isolated from C57BL/6 mice and carefully ground into single cells and then were cultured in RPMI 1640 medium supplemented with 10% FBS, 100 U/mL of penicillin, 100 mg/mL of streptomycin, soluble anti-CD3 (5 μg mL−1; 16– 0031-86; eBioscience/Thermo Fisher Scientific, Waltham, MA), anti-CD28 (5 μg mL−1; 16–0281-86; eBioscience) and IL-2 (200 U mL−1; 212–12; Peprotech, NJ, USA) for 3 days.25,26 The chemotaxis assay was then performed in 24-well plates with Transwell permeable supports using a 5 μm polycarbonate membrane (3421; Corning Life Sciences, Tewksbury, MA, USA). .. Briefly, 500 μL of 1640 alone, the supernatant of control adenovirus (Adv-Ctrl)- or Adv-CXCL10-infected MC38-CAR or 1640 with 200 pg/mL of recombinant murine CXCL10 (rCXCL10; 250–16; Peprotech), all of which were supplemented with 10% FBS, were added to the lower well. ..

    Control:

    Article Title: Islet‐Resident Memory T Cells Orchestrate the Immunopathogenesis of Type 1 Diabetes through the FABP4‐CXCL10 Axis
    Article Snippet: Activated CD8 + T cells (1 × 10 5 cells/well) were then seeded on the porous permeable membrane in the Trans‐well Supports (Corning). .. To assess the alarming function of FABP4 +/+ T RM and FABP4 −/− T RM cells and the involvement of CXCL10, the conditioned medium of respective cells were added to the lower wells. and supplemented with recombinant murine CXCL10 (2 g mL −1 , PeproTech) or IgG as control. ..

    Article Title: Islet-Resident Memory T Cells Orchestrate the Immunopathogenesis of Type 1 Diabetes through the FABP4-CXCL10 Axis.
    Article Snippet: Activated CD8+ T cells (1 × 105 cells/well) were then seeded on the porous permeable membrane in the Trans-well Supports (Corning). .. To assess the alarming function of FABP4+/+ TRM and FABP4−/− TRM cells and the involvement of CXCL10, the conditioned medium of respective cells were added to the lower wells. and supplemented with recombinant murine CXCL10 (2 g mL−1, PeproTech) or IgG as control. ..

    Article Title: CXCL10-armed oncolytic adenovirus promotes tumor-infiltrating T-cell chemotaxis to enhance anti-PD-1 therapy
    Article Snippet: Lymph nodes were isolated from C57BL/6 mice and carefully ground into single cells and then were cultured in RPMI 1640 medium supplemented with 10% FBS, 100 U/mL of penicillin, 100 mg/mL of streptomycin, soluble anti-CD3 (5 μg mL −1 ; 16–0031-86; eBioscience/Thermo Fisher Scientific, Waltham, MA), anti-CD28 (5 μg mL −1 ; 16–0281-86; eBioscience) and IL-2 (200 U mL −1 ; 212–12; Peprotech, NJ, USA) for 3 days., The chemotaxis assay was then performed in 24-well plates with Transwell permeable supports using a 5 μm polycarbonate membrane (3421; Corning Life Sciences, Tewksbury, MA, USA). .. Briefly, 500 μL of 1640 alone, the supernatant of control adenovirus (Adv-Ctrl)- or Adv-CXCL10-infected MC38-CAR or 1640 with 200 pg/mL of recombinant murine CXCL10 (rCXCL10; 250–16; Peprotech), all of which were supplemented with 10% FBS, were added to the lower well. ..

    Article Title: CXCL10-armed oncolytic adenovirus promotes tumor-infiltrating T-cell chemotaxis to enhance anti-PD-1 therapy
    Article Snippet: Isolation and culture of murine lymphocytes and the in vitro chemotaxis assay Lymph nodes were isolated from C57BL/6 mice and carefully ground into single cells and then were cultured in RPMI 1640 medium supplemented with 10% FBS, 100 U/mL of penicillin, 100 mg/mL of streptomycin, soluble anti-CD3 (5 μg mL−1; 16– 0031-86; eBioscience/Thermo Fisher Scientific, Waltham, MA), anti-CD28 (5 μg mL−1; 16–0281-86; eBioscience) and IL-2 (200 U mL−1; 212–12; Peprotech, NJ, USA) for 3 days.25,26 The chemotaxis assay was then performed in 24-well plates with Transwell permeable supports using a 5 μm polycarbonate membrane (3421; Corning Life Sciences, Tewksbury, MA, USA). .. Briefly, 500 μL of 1640 alone, the supernatant of control adenovirus (Adv-Ctrl)- or Adv-CXCL10-infected MC38-CAR or 1640 with 200 pg/mL of recombinant murine CXCL10 (rCXCL10; 250–16; Peprotech), all of which were supplemented with 10% FBS, were added to the lower well. ..



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    T RM cells enhance the immunopathologic development of T1D through the FABP4‐CXCL10 axis. A) Representative immunoblots of CXCL10 and HSP90 in the pancreas of FABP4 +/+ NOD and FABP4 −/− NOD mice. The right panel is the quantification of the band intensity of CXCL10 relative to HSP90 ( n = 4). B) The mRNA abundance of FABP4, IFNγ, and CXCL10 in FABP4 +/+ T RM cells and FABP4 −/− T RM cells isolated from NOD mice ( n = 6). C) Representative FACS plots showing the abundance of IFNγ and CXCL10 in FABP4 +/+ T RM cells or FABP4 −/− T RM cells. The right panel is the quantification of positive cell percentage ( n = 5). D) Concentration of IFNγ and CXCL10 in the medium of FABP4 +/+ T RM cells or FABP4 −/− T RM cells stimulated with CD3 + CD28 + beads or vehicle ( n = 6). E,F) Representative images (E) and quantification (F) of displacement length (left) and average speed (right) analyzed by Imaris showing the motility of GFP‐labeled CD8 + T cells cultured in the conditioned media of FABP4 +/+ T RM or FABP4 −/− T RM cells in the presence or absence of recombinant CXCL10 protein (2 µg mL −1 ) or IgG control (2 µg mL −1 ) for 24 h (scale bar 50 µm). G) The number of residual (upper) and migrated (lower) CD8 + T cells cultured in the trans‐well plate with a conditioned medium of FABP4 +/+ T RM cells or FABP4 −/− T RM cells in the presence or absence of recombinant CXCL10 protein (2 µg mL −1 ) or IgG (2 µg mL −1 ) for 24 h ( n = 3). H,I) Representative pictures (H) and quantification (I) of displacement length (left) and average speed (right) showing the motility of GFP‐labeled CD8 + T cells cultured in the conditioned medium of FABP4 +/+ T RM or FABP4 −/− T RM cells treated with siCXCL10 (5 nmol) or siControl (siCtrl, 5 nmol) for 24 h (scale bar 50 µm). J) The number of residual (upper) and migrated (lower) CD8 + T cells cultured in the trans‐well plate with the conditioned medium from FABP4 +/+ T RM cells or FABP4 −/− T RM cells treated with siCXCL10 or siCtrl (5 nmol) for 24 h ( n = 5). Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Unpaired Student's t test or Mann‐Whitney U test was used in (A), (B), and (C). ANOVA followed by Sidak's test was used in (D), (F), (G), (I), and (J).

    Journal: Advanced Science

    Article Title: Islet‐Resident Memory T Cells Orchestrate the Immunopathogenesis of Type 1 Diabetes through the FABP4‐CXCL10 Axis

    doi: 10.1002/advs.202308461

    Figure Lengend Snippet: T RM cells enhance the immunopathologic development of T1D through the FABP4‐CXCL10 axis. A) Representative immunoblots of CXCL10 and HSP90 in the pancreas of FABP4 +/+ NOD and FABP4 −/− NOD mice. The right panel is the quantification of the band intensity of CXCL10 relative to HSP90 ( n = 4). B) The mRNA abundance of FABP4, IFNγ, and CXCL10 in FABP4 +/+ T RM cells and FABP4 −/− T RM cells isolated from NOD mice ( n = 6). C) Representative FACS plots showing the abundance of IFNγ and CXCL10 in FABP4 +/+ T RM cells or FABP4 −/− T RM cells. The right panel is the quantification of positive cell percentage ( n = 5). D) Concentration of IFNγ and CXCL10 in the medium of FABP4 +/+ T RM cells or FABP4 −/− T RM cells stimulated with CD3 + CD28 + beads or vehicle ( n = 6). E,F) Representative images (E) and quantification (F) of displacement length (left) and average speed (right) analyzed by Imaris showing the motility of GFP‐labeled CD8 + T cells cultured in the conditioned media of FABP4 +/+ T RM or FABP4 −/− T RM cells in the presence or absence of recombinant CXCL10 protein (2 µg mL −1 ) or IgG control (2 µg mL −1 ) for 24 h (scale bar 50 µm). G) The number of residual (upper) and migrated (lower) CD8 + T cells cultured in the trans‐well plate with a conditioned medium of FABP4 +/+ T RM cells or FABP4 −/− T RM cells in the presence or absence of recombinant CXCL10 protein (2 µg mL −1 ) or IgG (2 µg mL −1 ) for 24 h ( n = 3). H,I) Representative pictures (H) and quantification (I) of displacement length (left) and average speed (right) showing the motility of GFP‐labeled CD8 + T cells cultured in the conditioned medium of FABP4 +/+ T RM or FABP4 −/− T RM cells treated with siCXCL10 (5 nmol) or siControl (siCtrl, 5 nmol) for 24 h (scale bar 50 µm). J) The number of residual (upper) and migrated (lower) CD8 + T cells cultured in the trans‐well plate with the conditioned medium from FABP4 +/+ T RM cells or FABP4 −/− T RM cells treated with siCXCL10 or siCtrl (5 nmol) for 24 h ( n = 5). Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. Unpaired Student's t test or Mann‐Whitney U test was used in (A), (B), and (C). ANOVA followed by Sidak's test was used in (D), (F), (G), (I), and (J).

    Article Snippet: To assess the alarming function of FABP4 +/+ T RM and FABP4 −/− T RM cells and the involvement of CXCL10, the conditioned medium of respective cells were added to the lower wells. and supplemented with recombinant murine CXCL10 (2 g mL −1 , PeproTech) or IgG as control.

    Techniques: Western Blot, Isolation, Concentration Assay, Labeling, Cell Culture, Recombinant, Control, MANN-WHITNEY

    Targeting FABP4 resembles T RM cell depletion in alleviating T1D development. FABP4 +/+ NOD and FABP4 −/− NOD mice were subjected to the treatment of anti‐CD69 neutralizing antibody to deplete T RM cells. A) Schematic diagram showing the protocol of T RM cell depletion in NOD mice. Six‐week‐old female FABP4 +/+ NOD and FABP4 −/− NOD mice were injected with anti‐CD69 antibody (40 µg/mouse) or IgG every week (i.v.) for 8 weeks ( n = 5). B) The representative FACS plots show the abundance of T RM cells (CD8 + CD44 + CD69 + CD62L − ) in the mouse pancreas. The right panel is the quantification of the percentage of T RM cells ( n = 5). C) Diabetes incidence of mice ( n = 5). D) Scoring of histological grades of insulitis in mice ( n = 5). E) Representative images of immunofluorescent co‐staining for insulin‐positive β cells (green) and TUNEL‐positive apoptotic cells (red) in mouse pancreas (scale bar 20 µm). The lower panel is the quantification of the number of apoptotic cells within the islet area ( n = 5). F) The mRNA abundance of inflammatory cytokines and chemokines in mouse pancreas ( n = 5). G) The abundance of CXCL10 protein in mouse pancreatic islets ( n = 4). H) Representative immunoblots of CXCL10 and HSP90 in mouse pancreas. The lower panel is the quantification of the band intensity of CXCL10 relative to HSP90 ( n = 4). I) The abundance of total cytotoxic T cells (CD8 + CD44 + ), antigen‐experienced T cells (CD8 + CXCR3 + ), and IFN γ or Granzyme B expressing CD8 + T cells infiltrated in the pancreatic islets of the above mice ( n = 5). Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. ANOVA followed by Sidak's test was used in (B), (E), (F), (G), (H), and (I). The log‐rank test was used in (C). The Pearson's chi‐square test was used in (D).

    Journal: Advanced Science

    Article Title: Islet‐Resident Memory T Cells Orchestrate the Immunopathogenesis of Type 1 Diabetes through the FABP4‐CXCL10 Axis

    doi: 10.1002/advs.202308461

    Figure Lengend Snippet: Targeting FABP4 resembles T RM cell depletion in alleviating T1D development. FABP4 +/+ NOD and FABP4 −/− NOD mice were subjected to the treatment of anti‐CD69 neutralizing antibody to deplete T RM cells. A) Schematic diagram showing the protocol of T RM cell depletion in NOD mice. Six‐week‐old female FABP4 +/+ NOD and FABP4 −/− NOD mice were injected with anti‐CD69 antibody (40 µg/mouse) or IgG every week (i.v.) for 8 weeks ( n = 5). B) The representative FACS plots show the abundance of T RM cells (CD8 + CD44 + CD69 + CD62L − ) in the mouse pancreas. The right panel is the quantification of the percentage of T RM cells ( n = 5). C) Diabetes incidence of mice ( n = 5). D) Scoring of histological grades of insulitis in mice ( n = 5). E) Representative images of immunofluorescent co‐staining for insulin‐positive β cells (green) and TUNEL‐positive apoptotic cells (red) in mouse pancreas (scale bar 20 µm). The lower panel is the quantification of the number of apoptotic cells within the islet area ( n = 5). F) The mRNA abundance of inflammatory cytokines and chemokines in mouse pancreas ( n = 5). G) The abundance of CXCL10 protein in mouse pancreatic islets ( n = 4). H) Representative immunoblots of CXCL10 and HSP90 in mouse pancreas. The lower panel is the quantification of the band intensity of CXCL10 relative to HSP90 ( n = 4). I) The abundance of total cytotoxic T cells (CD8 + CD44 + ), antigen‐experienced T cells (CD8 + CXCR3 + ), and IFN γ or Granzyme B expressing CD8 + T cells infiltrated in the pancreatic islets of the above mice ( n = 5). Data are expressed as mean ± SD. * p < 0.05, ** p < 0.01, *** p < 0.001. ANOVA followed by Sidak's test was used in (B), (E), (F), (G), (H), and (I). The log‐rank test was used in (C). The Pearson's chi‐square test was used in (D).

    Article Snippet: To assess the alarming function of FABP4 +/+ T RM and FABP4 −/− T RM cells and the involvement of CXCL10, the conditioned medium of respective cells were added to the lower wells. and supplemented with recombinant murine CXCL10 (2 g mL −1 , PeproTech) or IgG as control.

    Techniques: Injection, Staining, TUNEL Assay, Western Blot, Expressing

    T RM cells orchestrate the immunopathogenesis of type 1 Diabetes through FABP4‐CXCL10 axis.

    Journal: Advanced Science

    Article Title: Islet‐Resident Memory T Cells Orchestrate the Immunopathogenesis of Type 1 Diabetes through the FABP4‐CXCL10 Axis

    doi: 10.1002/advs.202308461

    Figure Lengend Snippet: T RM cells orchestrate the immunopathogenesis of type 1 Diabetes through FABP4‐CXCL10 axis.

    Article Snippet: To assess the alarming function of FABP4 +/+ T RM and FABP4 −/− T RM cells and the involvement of CXCL10, the conditioned medium of respective cells were added to the lower wells. and supplemented with recombinant murine CXCL10 (2 g mL −1 , PeproTech) or IgG as control.

    Techniques: